During the second stage of the project No. 18.104.22.168/16/A/258 “Development and the application of innovative instrumental analytical methods for the combined determination of a wide range of chemical and biological contaminants in support of the bio-economy in the priority sectors of economy” work was continued on the development of chemical and biological methods for the detection of contaminants in various biota and food samples.
Studies have been carried out on mycotoxin detection tests with flattening mass spectrometry and on-line solid-phase extraction technology (online-SPE). In addition to the 12 existing reference substances, five more new mycotoxin reference substances were received, and immediately added to the range of mycotoxins to be analyzed. Currently, 17 instrumental detection HPLC-qTOF methods for mycotoxins have been developed in the framework of the study.
he UHPLC-q-Orbitrap method was approbated and validated for the determination of environmental contaminants (brominated flame retardants) in eel samples and wild game meat.
n the second stage, studies were carried out on the determination of coccidiosts by the HPLC-q-Orbitrap method, using optimization of the method with the design of experiments (DoE) approach. This approach allows to achieve the best method parameters with the smallest number of experiments.
uring the second phase of the project the development of biologic pollutant methods have been continued. Researchers compared MALDI-TOF and PCR molecular methods and concluded that Aspergillus niger (22%), A. flavus (17%) and A. versicolor (15%) species dominated in more than 200 mold isolates.
The PCR method and sequencing analysis have been used to improve the detection of molds and to increase the detection sensitivity of food pathogens.
Within the project microbial and molecular detection methods of sporulating bacteria Bacillus cereus have been compared also.
In order to ensure the transfer of knowledge, two publications are submitted in the journals and currently the publications review are ongoing.
The total project costs: EUR 449, 617.00 (the planned ERDF aid (85%): EUR 382, 174, 44 and state budget aid (7, 5%): EUR33721, 28). Implementation of the project is scheduled at the duration of thirty months (period: 01.03.2017. - 30.08. 2019).
Information prepared on 26.09.2017.