Blood serum, milk
Manufacturer's methodology, Enzyme-linked immunosorbent assay (ELISA)
Blood - not less than 3 ml, in vacuum test tube;
Milk - not less than 30 ml in single-use plastic containers or test tubes that have been certified for packaging biological products with screwable or closely closable cover
Milk, tissue sample of respiratory organs, joint tissues and fluid, nasal swab
Real time Polymerase Chain Reaction - Clothier et al. (2010)
Not less than 10 g of tissues or milk, nasal swab, container or in double package (+5±3) °C
Tracheal swab, sample from tissue of respiratory organs, pleural fluid, lymph nodes, synovial fluid (for animals with signs of arthritis), EDTA stabilized blood, semen, urine;
EDTA stabilized blood (for cats only)
Polymerase Chain Reaction (PCR)
Nasal or oro-faringeal swab, bronchoalveolar fluid, suspensions of respiratory system tissues, lung, trachea - not less than 1 ml stabilized blood in vacuum test tube, urine, semen in sterile container (+5±3) °C