Blood serum, plasma
OIE Manual, chapter 2.1.3., Enzyme-linked immunosorbent assay (ELISA)
Not less than 3 ml in vacuum test tube
Milk
OIE Manual, chapter 2.1.3., Enzyme-linked immunosorbent assay (ELISA)
Milk - not less than 30 ml in single-use plastic containers or test tubes that have been certified for packaging biological products with screwable or closely closable cover
Virus isolates, clinical or tissue samples
Sequencing reaction
Virus containing suspension prepared from clinical or tissue samples; virus isolates after multiplication in chicken embryos or cell cultures under laboratory circumstances (+5±3) °C
Approval of the serotype in the situation of a repeated outbreak
EDTA stabilised blood, pathological material, animal tissues (ruminant), virus RNA released
Sequencing reaction
Parenhimatose organs, fresh or fresh-frozen, small animals or aborted fetus, all corpse, EDTA blood (not less than 3 ml), +5±3 °C.
Organs should be packaged separately in densely enclosed containers, Petri plates and waterproof packaging. Samples from one animal should be placed in additional packaging.
Differentiation of the 5 most common serotypes
EDTA stabilised blood, pathological material, animal tissues (ruminant), virus RNA released
Sequencing reaction
Parenhimatose organs, fresh or fresh-frozen, small animals or aborted fetus, all corpse, EDTA blood (not less than 3 ml), +5±3 °C.
Organs should be packaged separately in densely enclosed containers, Petri plates and waterproof packaging. Samples from one animal should be placed in additional packaging.